Cell line/type | γδ (gammadelta) T-cells |
---|---|
Species | Human |
Animal free | Yes |
Product | OpTmizer |
Sutton, K. S., Dasgupta, A., McCarty, D., Doering, C. B., & Spencer, H. T. (2016). Bioengineering and serum free expansion of blood-derived γδ T cells. Cytotherapy, 18(7), 881-892. In this study, gammadelta T-cell expansion was evaluated in several serum-free media (SFM). Additionally, the expanded gammadelta T cells were evaluated for their transduction efficiency using lentiviral vectors (LV). Of the SFM cultures, robust expansion was only observed in OpTmizer supplemented with high-dose interleukin-2. gammadelta T-cell percentages and numbers were sufficient for clinical use. Using cells from several donors, transduction efficiencies ranged from 13 to 33%, which is similar to transduction levels observed using alphabeta T cells with similar multiplicity of infection. The use of SFM provides clinically safer, widely applicable and potentially more efficacious cellular immunotherapy. Cells were cultured in OpTmizer supplemented with high-dose interleukin-2. OpTmizer is a commercial product. The formulation is proprietary. https://www.sciencedirect.com/science/article/pii/S1465324916303437?via%3Dihub |
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Source | Literature - modified commercial product |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |