Cell line/type | RTgill-W1 |
---|---|
Species | Rainbow trout (Oncorhynchus mykiss) |
Animal free | Yes |
Product | InVitrus VP-6 |
Yue, Y., Behra, R., Sigg, L., & Schirmer, K. (2016). Silver nanoparticles inhibit fish gill cell proliferation in protein-free culture medium. Nanotoxicology, 10(8), 1075-1083. In this study, the rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1, was adapted to proliferate in a commercial, serum-free medium, InVitrus VP-6. The newly adapted cell strain was named RTgill-W1-pf (protein free). These cells proliferate at a speed similar to the RTgill-W1 cells cultured in a fully supplemented medium containing 5% fetal bovine serum. As well, they were successfully cryopreserved in liquid nitrogen and fully recovered after thawing. Yet, senescence set in after about 10 passages in InVitrus VP-6 medium, revealing that this medium cannot fully support long-term culture of the RTgill-W1 strain. InVitrus VP-6 medium is a commercial product. The formulation is proprietary. https://www.tandfonline.com/doi/pdf/10.3109/17435390.2016.1172677?needAccess=true |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |