Cell line/type | HEK293, Expi293F |
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Species | Human |
Animal free | Yes |
Product | Expi293 medium |
Adamson-Small, L., Potter, M., Byrne, B. J., & Clement, N. (2017). Sodium chloride enhances recombinant adeno-associated virus production in a serum-free suspension manufacturing platform using the herpes simplex virus system. Human gene therapy methods, 28(1), 1-14. This study reports production and optimization of rAAV using the rHSV system in suspension HEK293 cells (Expi293F) grown in serum and animal component-free medium. Through adjustment of salt concentration in the medium and optimization of infection conditions, titers greater than 1 x 1014 vector genomes per liter (VG/liter) were observed in purified rAAV stocks produced in Expi293F cells. Furthermore, this system allowed for high-titer production of multiple rAAV serotypes (2, 5, and 9) as well as multiple transgenes (green fluorescent protein and acid alpha-glucosidase). A proportional increase in vector production was observed as this method was scaled, with a final 3-liter shaker flask production yielding an excess of 1 x 1015 VG in crude cell harvests and an average of 3.5 x 1014 total VG of purified rAAV9 material, resulting in greater than 1 x 105 VG/cell. These results support the use of this rHSV-based rAAV production method for large-scale preclinical and clinical vector production. Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium. Expi293™ Expression Medium is a proprietary, commercial medium. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346630/pdf/hgtb.2016.151.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |