Cell line/type | OACs (osteoarthritic articular chondrocytes) |
---|---|
Species | Human |
Animal free | Yes |
Product | CM high glucose DMEM Bundens et al. 2017 |
Bundens, G., Buckley, A., Milton, L., Behling, K., Chmielewski, S., Cho, E., ... & Miller, L. (2017). Measuring clinically relevant endpoints in a serum-free, three-dimensional, primary cell culture system of human osteoarthritic articular chondrocytes. Experimental cell research, 357(2), 310-319. The purpose of this study was to develop a primary, serum-free cell culture system of human osteoarthritic articular chondrocytes (HOACs) with which to study manifestations of the disease process. HOACs isolated from the femoral condyles and tibial plateau of the same side were combined, plated in three-dimensional, alginate beads and cultured for five days in serum, hormone and protein-free medium. This serum-free, three-dimensional primary culture system of HOACs provides a platform with which to measure clinically relevant endpoints of OA and screen potential disease modifying OA therapeutics. Cells were cultured in conditioned medium collected from cells cultured in serum-free, high glucose DMEM medium. See Methods, 2.3. Preparation of primary cultures of HOACs for preparation details of the conditioned medium. https://www.sciencedirect.com/science/article/pii/S0014482717303300 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |