Cell line/type | LESCs (Limbal epithelial stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | XF-medium |
Lužnik, Z., Breda, C., Barbaro, V., Ferrari, S., Migliorati, A., Di Iorio, E., ... & Bertolin, M. (2017). Towards xeno-free cultures of human limbal stem cells for ocular surface reconstruction. Cell and tissue banking, 18(4), 461-474. Isolated limbal epithelial stem cells (LESCs) were cultured with or without a 3T3 murine fibroblast feeder-layer (FL) in 4 different culture media on culture plates or on denuded human amniotic membrane (AM) support and fibrin gel support: (1) control medium supplemented with fetal bovine serum; (2) control medium supplemented with the synthetic serum "XerumFree XF205" (XF); (3) CnT-20 medium supplemented with "XerumFree XF205" (CnT-XF) and (4) CnT-20 medium supplemented with human AB serum (CnT-AB).During serial cultivation of LESCs, most of the tested xeno-free media supported similar numbers of cell passages, total colony number, cumulative cell doublings (CCD) rates and expression of Np63alpha compared to control. The conditions cultivated with a FL showed a non-statistically significant higher number of cell passages and CCD rates before senescence when compared to the same conditions cultured without FL. Except for the control medium, only XF medium enabled the growth of cells on AM. The expression of Np63alpha was comparable in all the cultures grown onto AM, when compared to the controls on fibrin gel. In conclusion, the xeno-free media enabled LESC culture both on plastic and on denuded human AM. XF-Medium is the control medium with 10% XerumFreeTM XF205, as specified in the materials and methods. The formulation of the control medium is listed in the Materials and Methods, under the heading; Cell culture media. https://link.springer.com/content/pdf/10.1007%2Fs10561-017-9632-7.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |