Cell line/type | Scalp skin |
---|---|
Species | Human |
Animal free | Yes |
Product | William’s E medium Lu et al. 2007 |
Lu, Z., Hasse, S., Bodo, E., Rose, C., Funk, W., & Paus, R. (2007). Towards the development of a simplified long‐term organ culture method for human scalp skin and its appendages under serum‐free conditions. Experimental dermatology, 16(1), 37-44. In this study, is reported that intact human scalp skin can be grown at the air/liquid interface in supplemented, serum-free William's E medium for more than 2 weeks. Active hair shaft growth was visible until day 16 and was significantly enhanced compared with minimum essential medium (MEM) + 10% fetal bovine serum (FBS). Moreover, William's E medium protected better against cell death than MEM + 10% FBS before day 12. The preliminary data suggest that a very simple serum-free organ culture method allows prolonged human skin and hair follicle survival as well as some limited hair follicle cycling in intact skin for more than 2 weeks under well-defined experimental conditions. William’s E medium was supplemented with different components as specified in the Materials and Methods, under the heading; Preparation and histoculture of human scalp skin. The formulation of William’s E medium can be found online: https://www.sigmaaldrich.com/life-science/cell-culture/learning-center/media-formulations/williams.html https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0625.2006.00510.x |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |