Cell line/type | Renal tubules |
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Species | Rabbit |
Animal free | Yes |
Product | IMDM Heber et al. 2007 |
Heber, S., Denk, L., Hu, K., & Minuth, W. W. (2007). Modulating the development of renal tubules growing in serum-free culture medium at an artificial interstitium. Tissue engineering, 13(2), 281-292. In this study, an advanced culture method to follow tubule development was developed. Isolated tissue containing renal progenitor cells was placed in a perfusion culture container at the interphase of an artificial polyester interstitium. Iscove's modified Dulbecco's medium without serum or protein supplementation was used for culture, and the culture period was 13 days. Tissue growth was not supported by addition of extracellular matrix proteins. The development of tubules was registered on cryosections labeled with soybean agglutinin (SBA) and tissue-specific antibodies. Multiple SBA-labeled tubules were found when aldosterone was added to the culture medium. In contrast, culture without aldosterone supplementation displayed completely disintegrated tissue. The development of tubules depended on the applied aldosterone concentration. The use of 1 x 10(-6) M and 1 x 10(-7) M aldosterone produced numerous tubules, while application of 1 x 10(-8) M to 1 x 10(-10) M led to a continuous decrease and finally a loss of tubule formation. IMDM medium was supplemented with different components as specified in the Materials and Methods, under the heading; 2.2. Perfusion culture of renal tubules at the interphase of an artificial interstitium. https://www.liebertpub.com/doi/abs/10.1089/ten.2006.0199 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |