Cell line/type | Renal tubules |
---|---|
Species | Rabbit |
Animal free | Yes |
Product | IMDM Minuth et al. 2007 |
Minuth, W. W., Denk, L., & Hu, K. (2007). The role of polyester interstitium and aldosterone during structural development of renal tubules in serum-free medium. Biomaterials, 28(30), 4418-4428. In this study, embryonic stem/progenitor cells derived from neonatal rabbit kidney were placed in a perfusion culture container at the interphase of an artificial polyester interstitium. Tissue culture was carried out in IMDM without serum or protein supplementation and without coating with extracellular matrix proteins. Development of tubules was registered histochemically on cryosections labeled with soybean agglutinin (SBA) and tissue-specific antibodies. The experiments revealed that the development of renal tubules depends exclusively on the administration of aldosterone. The use of 1x10(-7) M aldosterone for 13 days generated numerous SBA-labeled tubules, while no tubules developed in the absence of the steroid hormone. Application of cholesterol, pregnenolone, progesterone, 11-deoxycorticosterone (DOCA) and corticosterone failed to form numerous tubules. Only 11-DOCA and progesterone induced a few tubules, which were barely SBA-labeled. Furthermore, application of aldosterone antagonists such as 1x10(-4) M spironolactone and 1x10(-4) M canrenoate completely inhibited the development of tubules. The authors conclude that specifically aldosterone promotes the development of tubules via the mineralocorticoid receptor whereas its precursors have no effect. IMDM medium was supplemented with different components as specified in the Materials and Methods, under the heading; 2.2. Perfusion culture of renal tubules at the interphase of an artificial interstitium. https://www.sciencedirect.com/science/article/pii/S0142961207005054 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |