Meneses-Acosta, A., Dormond, E., Jacob, D., Tom, R., Bernier, A., Perret, S., ... & Kamen, A. (2008). Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions. Journal of virological methods, 148(1-2), 106-114.
DOI: 10.1016/j.jviromet.2007.10.017

In this work, a scalable system for HDAd production is designed and evaluated focusing on the co-infection step. A human embryo kidney 293 (293) derived cell line, the 293SF/FLPe was generated to produce efficiently HDAd while restricting the packaging of HAd. The best FLPe clone was adapted to grow in serum-free NSFM13 supplemented with 0.75 µg/mL of puromycin. This new cell line is referred to as 293SF/FLPe cell line.

Serum-free NSFM13 medium was supplemented with different components as specified in the Materials and Methods, under the heading; 2.2. Cell lines and culture media. The preperation of NSFM13m medium is described in a previous article, Nadeau et al. 2002. 
Nadeau, I., Gilbert, P.A., Jacob, D., Perrier, M., Kamen, A., 2002. Low-protein medium affects the 293SF central metabolism during growth and infection with adenovirus. Biotechnol. Bioeng. 77, 91–104.