Cell line/type | 293SF/FLPe (human embryo kidney derived) |
---|---|
Species | Human |
Animal free | Unspecified |
Product | NSFM13-puromycin |
Meneses-Acosta, A., Dormond, E., Jacob, D., Tom, R., Bernier, A., Perret, S., ... & Kamen, A. (2008). Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions. Journal of virological methods, 148(1-2), 106-114. In this work, a scalable system for HDAd production is designed and evaluated focusing on the co-infection step. A human embryo kidney 293 (293) derived cell line, the 293SF/FLPe was generated to produce efficiently HDAd while restricting the packaging of HAd. The best FLPe clone was adapted to grow in serum-free NSFM13 supplemented with 0.75 µg/mL of puromycin. This new cell line is referred to as 293SF/FLPe cell line. Serum-free NSFM13 medium was supplemented with different components as specified in the Materials and Methods, under the heading; 2.2. Cell lines and culture media. The preperation of NSFM13m medium is described in a previous article, Nadeau et al. 2002. |
|
Source | Literature - modified commercial product |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |