|Cell line/type||293SF/FLPe (human embryo kidney derived)|
Meneses-Acosta, A., Dormond, E., Jacob, D., Tom, R., Bernier, A., Perret, S., ... & Kamen, A. (2008). Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions. Journal of virological methods, 148(1-2), 106-114.
In this work, a scalable system for HDAd production is designed and evaluated focusing on the co-infection step. A human embryo kidney 293 (293) derived cell line, the 293SF/FLPe was generated to produce efficiently HDAd while restricting the packaging of HAd. The best FLPe clone was adapted to grow in serum-free NSFM13 supplemented with 0.75 µg/mL of puromycin. This new cell line is referred to as 293SF/FLPe cell line.
Serum-free NSFM13 medium was supplemented with different components as specified in the Materials and Methods, under the heading; 2.2. Cell lines and culture media. The preperation of NSFM13m medium is described in a previous article, Nadeau et al. 2002.
|Source||Literature - modified commercial product|
|Chemically defined > Unspecified||Unspecified|
|Contains phenol red > Yes||Yes|
|Antibiotics free > No||No|