Cell line/type | Hybridoma 16G9 |
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Species | Hybridoma |
Animal free | Yes |
Product | CDHybridoma medium |
Shimada, Y., Goto, T., Kawamoto, S., Kiso, T., Katayama, A., Yamanaka, Y., ... & Chen, C. L. (2008). Development of a two‐step chromatography procedure that allows the purification of a high‐purity anti‐histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Biomedical Chromatography, 22(1), 13-19. In this study, hybridomas that produce anti-histone H1 mAb were prepared with immunosuppressant activity and a two-step purification procedure, consisting of strong anion-exchange chromatography and gel filtration chromatography was developed by which high-purity, 16G9 mAb administrable to organ transplantation model animals is purified from the serum-free culture supernatant. The monoclonal antibody was successfully purified at 96%, a purification rate at which its administration to organ transplantation model animals is possible. The serum-free CDHybridoma medium was supplemented with different components as specified in the Materials and Methods, under the heading; Production of 16G9 mAb in the serum-free culture. CDHybridoma medium is a commercial product. The formulation is proprietary. https://onlinelibrary.wiley.com/doi/pdf/10.1002/bmc.887 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |