|Cell line/type||Hybridoma 16G9|
Shimada, Y., Goto, T., Kawamoto, S., Kiso, T., Katayama, A., Yamanaka, Y., ... & Chen, C. L. (2008). Development of a two‐step chromatography procedure that allows the purification of a high‐purity anti‐histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Biomedical Chromatography, 22(1), 13-19.
In this study, hybridomas that produce anti-histone H1 mAb were prepared with immunosuppressant activity and a two-step purification procedure, consisting of strong anion-exchange chromatography and gel filtration chromatography was developed by which high-purity, 16G9 mAb administrable to organ transplantation model animals is purified from the serum-free culture supernatant. The monoclonal antibody was successfully purified at 96%, a purification rate at which its administration to organ transplantation model animals is possible.
The serum-free CDHybridoma medium was supplemented with different components as specified in the Materials and Methods, under the heading; Production of 16G9 mAb in the serum-free culture. CDHybridoma medium is a commercial product. The formulation is proprietary.https://onlinelibrary.wiley.com/doi/pdf/10.1002/bmc.887
|Source||Literature - modified commercial product|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > Yes||Yes|