Cell line/type | Skeletal muscle cells |
---|---|
Species | Mouse |
Animal free | No |
Product | DMEM/F10 Chaturvedi et al. 2015 |
Chaturvedi, V., Dye, D. E., Kinnear, B. F., van Kuppevelt, T. H., Grounds, M. D., & Coombe, D. R. (2015). Interactions between skeletal muscle myoblasts and their extracellular matrix revealed by a serum free culture system. PloS one, 10(6), e0127675. This study describes the efficient decellularisation of quadriceps muscle with the retention of matrix components and the use of this matrix for myoblast proliferation and differentiation under serum-free culture conditions. Under serum-free conditions, intact acellular muscle matrices provided cues to direct myoblast adhesion and migration. In addition, myoblasts were shown to rapidly secrete and organise their own matrix glycoproteins to create a localized ECM microenvironment. This serum-free culture system has revealed that the correct muscle ECM facilitates more rapid cell organisation and differentiation than single matrix glycoprotein substrates. The cells were cultured in DMEM/F10 supplemented with different components, as specified in the Materials and Methods, under the heading; Serum-free Proliferation and Differentiation assays. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450880/pdf/pone.0127675.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red | Yes |
Antibiotics free > Yes | Yes |