Cell line/type | Skeletal muscle cells |
---|---|
Species | Rat |
Animal free | Yes |
Product | SFM Das et al. 2007 |
Das, M., Wilson, K., Molnar, P., & Hickman, J. J. (2007). Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate. Nature protocols, 2(7), 1795. The protocol in this study describes a cell culture model to study the differentiation of fetal rat skeletal muscle cells. The model uses a serum-free medium, a nonbiological substrate N-1[3(trimethoxysilyl)propyl] diethylenetriamine (DETA) and fabricated microcantilevers to promote the differentiation of dissociated rat myocytes into robust myotubes. Here, four major techniques are employed: fabrication of cantilevers, surface modification of the glass and cantilever substrates with a DETA SAM, a serum-free medium and refined culture techniques. In this protocol, step-by-step instructions on how to fabricate and modify the cantilever and glass substrates and then grow the muscle cells on the chemically modified substrates using this serum-free medium, to form robust myotubes, is described. The precise formulation of the serum-free medium is listed in the Materials and Methods, under the heading; Reagent Setup, Serum-free medium. https://www.nature.com/articles/nprot.2007.229.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |