Cell line/type | Skeletal muscle cells |
---|---|
Species | Rat |
Animal free | Yes |
Product | SFM Das et al. 2009 |
Das, M., Rumsey, J. W., Bhargava, N., Gregory, C., Riedel, L., Kang, J. F., & Hickman, J. J. (2009). Developing a novel serum-free cell culture model of skeletal muscle differentiation by systematically studying the role of different growth factors in myotube formation. In Vitro Cellular & Developmental Biology-Animal, 45(7), 378-387. In this study, is the step-by-step development of a novel, serum-free, in vitro cell culture system described, resulting in the formation of robust, contracting, multinucleate myotubes from dissociated skeletal muscle cells obtained from the hind limbs of fetal rats. This defined system consisted of a serum-free medium formulation developed by the systematic addition of different growth factors as well as a nonbiological cell growth promoting substrate, N-1[3-(trimethoxysilyl) propyl] diethylenetriamine. Each growth factor in the medium was experimentally evaluated for its effect on myotube formation. The resulting myotubes were evaluated immunocytochemically using embryonic skeletal muscle, specifically the myosin heavy chain antibody. Based upon this analysis, a new skeletal muscle differentiation protocol was proposed that reflects the roles of the various growth factors which promote robust myotube formation. Further observation noted that the proposed skeletal muscle differentiation technique also supported muscle-nerve coculture. Immunocytochemical evidence of nerve-muscle coculture has also been documented. Applications for this novel culture system include biocompatibility and skeletal muscle differentiation studies, understanding myopathies, neuromuscular disorders, and skeletal muscle tissue engineering. The formulation of the novel serum-free medium is listed in Table 2. https://link.springer.com/content/pdf/10.1007%2Fs11626-009-9192-7.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > Yes | Yes |
Contains phenol red > Yes | Yes |