Cell line/type | Keratinocytes (cornea) |
---|---|
Species | Bovine (cow) |
Animal free | Yes |
Product | DMEM/F12 Kane at al. 2009 |
Kane, B. P., Jester, J. V., Huang, J., Wahlert, A., & Hassell, J. R. (2009). IGF-II and collagen expression by keratocytes during postnatal development. Experimental eye research, 89(2), 218-223. In this study, keratocytes were isolated from corneas of 6-8-week-old rabbits and corneas of 1-2-year-old cows and their ability to proliferate and synthesize collagen in serum-free media was determined. Rabbit keratocyte cultures increased 38% in DNA content after one week and deposited collagen type I and IGF-II in the media. Bovine keratocyte cultures, in contrast, did not increase in DNA or produce detectable collagen and IGF-II. Bovine keratocytes cultured in media previously conditioned by rabbit keratocytes, however, increased 56% in DNA content, and deposited collagen type I into the media. Microarray analysis of mRNA from neonatal and adult mouse keratocytes was used to confirm these differences. The formulation of the serum-free culture media, DMEM/F12 supplemented with different components, is specified in the Materials and Methods, under the heading 2.2. Isolation and culture of keratocytes. https://www.sciencedirect.com/science/article/pii/S0014483509000736 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |