Cell line/type | Keratinocytes (cornea) |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM/F12 + retinoic acid |
Gouveia, R. M., & Connon, C. J. (2013). The effects of retinoic acid on human corneal stromal keratocytes cultured in vitro under serum-free conditions. Investigative ophthalmology & visual science, 54(12), 7483-7491. The purpose of this study was to investigate the effects of retinoic acid (RA) on the phenotype of corneal stromal keratocytes maintained in vitro for extended periods under serum-free conditions. Keratocytes isolated from human corneas were cultured up to 21 days in serum-free media supplemented with RA or DMSO vehicle. Retinoic acid was shown to control the phenotype of human corneal keratocytes cultured in vitro by regulating cell behavior and extracellular matrix composition. The basal medium is DMEM/F12, supplemented with different components as specified in the Materials and Methods, under the heading; Cell culture. https://iovs.arvojournals.org/data/journals/iovs/932980/i1552-5783-54-12-7483.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |