Cell line/type | CHO (Chinese Hamster Ovary) C11-08 and H-1-5 |
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Species | Hamster |
Animal free | Yes |
Product | Basal SFM + hydrolysates |
Kim, S. H., & Lee, G. M. (2009). Development of serum-free medium supplemented with hydrolysates for the production of therapeutic antibodies in CHO cell cultures using design of experiments. Applied microbiology and biotechnology, 83(4), 639-648. In this study, an efficient method of formulating serum-free medium (SFM) for the production of therapeutic antibodies by recombinant CHO (rCHO) cells was developed using two rCHO cell lines producing a therapeutic antibody. In this method, ten kinds of SFM were prepared by supplementing the basal SFM with statistically designed mixtures (total 5 g L(-1)) of three non-animal-derived hydrolysates: yeastolate, soy hydrolysate, and wheat gluten hydrolysate. When the two rCHO cell lines were cultivated, the mixtures of soy hydrolysate and wheat gluten hydrolysate showed a positive effect on cell growth. On the other hand, the mixtures including a high portion of yeastolate significantly enhanced specific antibody productivity. To reconstitute the mixture ratios of the three hydrolysates for high growth and antibody production, the effect of each medium was analyzed by the statistical program Design-Expert(R). The resulting medium gave a 1.9-3.3-fold increase in the maximum antibody concentration, compared to the basal SFM. Both rCHO cell lines grew well in the basal SFM of which the individual component is listed in Table 1. This result suggests that the basal SFM is likely to support cell growth of most of rCHO cell lines. For both C11-08 and H-1-5 cells, mix S1, reconstituted for the highest maximum viable cell concentration, contains 60% of soy hydrolysate and 40% of wheat gluten hydrolysate. Mix S2, reconstituted for the highest antibody concentration, somewhat differed between the two cell lines. Mix S2 for C11-08 cells contains 52% of yeastolate, 18% of soy hydrolysate, and 30% of wheat gluten hydrolysate. For H-1-5 cells, mix S2 contains 70% of yeastolate and 30% of wheat gluten hydrolysate. The formulation of the basal serum-free medium is listed in Table 1 Composition of the basal SFM designed for rCHO cells. https://link.springer.com/content/pdf/10.1007%2Fs00253-009-1903-1.pdf |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |