Cell line/type | SGM (submandibular gland) epithelium |
---|---|
Species | Mouse |
Animal free | No |
Product | DMEM-F12/PS Rebusini et al. 2009 |
Rebustini, I. T., & Hoffman, M. P. (2009). ECM and FGF-dependent assay of embryonic SMG epithelial morphogenesis: investigating growth factor/matrix regulation of gene expression during submandibular gland development. In Extracellular Matrix Protocols (pp. 319-330). Humana Press. In this study, a detailed protocol for isolating primary embryonic SMG epithelium is described and an ECM and growth factor-dependent, serum-free assay of epithelial morphogenesis is set up, with subsequent analysis of gene expression by real-time PCR. Mouse embryonic submandibular gland (SMG) epithelium, isolated from its endogenous mesenchyme, undergoes branching morphogenesis when cultured ex vivo in a basement membrane extract in serum-free medium with growth factor stimulation. The resulting three-dimensional epithelial morphogenesis in the defined culture system makes this a useful model to analyze cell-cell and cell-matrix interactions, growth factor-mediated signaling and gene expression, proliferation, apoptosis, migration, lumen formation, and epithelial morphogenesis in a primary organ culture system. SMG epithelial culture is robust, reproducible, uses small amounts of reagents, and changes in gene expression are measured by real-time PCR using a limited amount of embryonic tissue. The formulation of the DMEM-F12/PS culture media is listed in the Materials 2.1. https://link.springer.com/content/pdf/10.1007%2F978-1-59745-413-1_21.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |