Cell line/type | Primary cortical neurons |
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Species | Mouse |
Animal free | No |
Product | Serum-free cell culture supplement |
Roth, S., Zhang, S., Chiu, J., Wirth, E. K., & Schweizer, U. (2010). Development of a serum-free supplement for primary neuron culture reveals the interplay of selenium and vitamin E in neuronal survival. Journal of Trace Elements in Medicine and Biology, 24(2), 130-137. In this study, a modified serum-free media supplement was developed which allows for free variation of all constituents. The supplement was comparable to B27 with regard to cell survival and expression of neurochemical markers. Reduction of Se content in the supplement reduced selenoprotein expression and made cortical neurons more sensitive towards challenges with peroxides. Withdrawal from the medium supplement of vitamin E alone did not alter the survival of neurons in response to peroxides, while simultaneous reduction of Se and vitamin E rendered neurons hypersensitive towards peroxide challenge. This finding implied that adequate Se supply of neurons is required to minimize lipid peroxidation. Our medium supplement is easily prepared, inexpensive, and should be applicable to the analysis of survival mechanisms beyond peroxide challenge. In Table 1 is the complete serum-free cell culture supplement formulation listed with final concentrations. Ingredients of this supplement are based on Brewer et al. [6,7] with some modifications. https://www.sciencedirect.com/science/article/pii/S0946672X10000088 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |