Cell line/type | Corneal epithelial cells |
---|---|
Species | Human |
Animal free | Yes |
Product | KGM and Pan+ |
Hahne, M., & Reichl, S. (2011). Development of a serum-free human cornea construct for in vitro drug absorption studies: the influence of varying cultivation parameters on barrier characteristics. International journal of pharmaceutics, 416(1), 268-279. This study describes the development of a serum-free cultivated, three-dimensional human cornea model (Hemicornea, HC) for drug absorption experiments.The impact of varying cultivation conditions on the corneal barrier function was analysed and compared with excised rabbit and porcine corneas.The most promising serum-free media, KGM and Pan+, were subsequently used for cultivating the Hemicornea construct. KGM and Pan+, were identified as appropriate for cultivating corneal epithelium. They resulted in TEER values between 1000 Ω cm2 and 2000 Ω cm2, which indicated barrier properties in the same range as those often described for excised rabbit corneas. The formulation of KGM and Pan+ are listed in Table 1 The cell-culture media and its compositions. https://www.sciencedirect.com/science/article/pii/S0378517311006338 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |