Cell line/type | SpESFX-10 (from myeloma cell line Sp2/0-Ag14 with Bcl2-EEE) |
---|---|
Species | Mouse |
Animal free | Unspecified |
Product | H-SFM |
Rossi, D. L., Rossi, E. A., Goldenberg, D. M., & Chang, C. H. (2011). A new mammalian host cell with enhanced survival enables completely serum‐free development of high‐level protein production cell lines. Biotechnology progress, 27(3), 766-775. In this study, the authors elected to transfect the myeloma cell line Sp2/0-Ag14 with Bcl2-EEE, the constitutively active phosphomimetic mutant of Bcl2, for extended cell survival. After adaptation of the initial transfectants to serum-independent growth, a clone with superior growth properties, referred to as SpESF, was isolated and further subjected to iterative rounds of stressful growth over a period of 4 months. The effort resulted in the selection of a promising clone, designated SpESFX-10, which was shown to exhibit robust growth and resist apoptosis induced by sodium butyrate or glutamine deprivation. The advantage of SpESFX-10 as a host for generating mAb-production cell lines was demonstrated by its increased transfection efficiency, culture longevity, and mAb productivity, as well as by the feasibility of accomplishing the entire cell line development process, including transfection, subcloning, and cryopreservation, in the complete absence of serum. Hybridoma Serum-Free Media (H-SFM) was supplemented with different components as specified in the Materials and Methods, under the heading; Cell culture. H-SFM is a commercial product. The precise formulation is proprietary. https://onlinelibrary.wiley.com/doi/pdf/10.1002/btpr.584 |
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Source | Literature - modified commercial product |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |