Cell line/type | BM-MSCs (Bone marrow-derived mesenchymal stromal cells) |
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Species | Human |
Animal free | No |
Product | BD Mosaic Mesenchymal Stem Cell Serum-free |
Gottipamula, S., Muttigi, M. S., Chaansa, S., Ashwin, K. M., Priya, N., Kolkundkar, U., ... & Seetharam, R. N. (2016). Large‐scale expansion of pre‐isolated bone marrow mesenchymal stromal cells in serum‐free conditions. Journal of tissue engineering and regenerative medicine, 10(2), 108-119. In this study, five different commercially available serum-free media (SFM) were screened for their ability to support the growth and expansion of pre-isolated undifferentiated bone marrow-derived MSCs (BM-MSCs) and compared the results with cells grown in standard FBS-containing medium as control. In addition, based on initial screening results, BD Mosaic Mesenchymal Stem Cell Serum-free (BD-SFM) medium was evaluated in large-scale cultures for the performance and culture characteristics of BM-MSCs. Of the five different serum-free media, BD-SFM enhanced BM-MSCs growth and expansion in Cell STACK (CS), but the cell yield per CS-10 was less when compared to the control medium. The characteristics of MSCs were measured in terms of population doubling time, cell yield and expression of MSC-specific markers. Significant differences were observed between BD-SFM and control medium in terms of population doublings, cell yield, CFU-F and morphological features, whereas surface phenotype and differentiation potentials were comparable. The BD-SFM-cultured MSCs were also found to retain the differentiation potential, immune-privileged status and immunosuppressive properties inherent to MSCs. The results suggest that BD-SFM supports large-scale expansion of BM-MSCs for therapeutic use. BD Mosaic Mesenchymal Stem Cell Serum-free is a proprietary, commercial product. https://onlinelibrary.wiley.com/doi/abs/10.1002/term.1713 |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |