Cell line/type | BM-MSCs (Bone marrow-derived mesenchymal stromal cells) |
---|---|
Species | Porcine (Pig) |
Animal free | No |
Product | ultra-CULTURE-EGF |
Wang, X., Zheng, F., Liu, O., Zheng, S., Liu, Y., Wang, Y., ... & Zhong, L. (2013). Epidermal growth factor can optimize a serum-free culture system for bone marrow stem cell proliferation in a miniature pig model. In Vitro Cellular & Developmental Biology-Animal, 49(10), 815-825. This study demonstrated that 10 nM/L EGF was the optimal dose for serum-free culture, which can replace traditional standard serum medium for in vitro expansion of miniature pig bone marrow-derived mesenchymal stem cells. Serum-free cultures were randomly divided and treated them with different levels of epidermal growth factor (EGF). Changes in rates of cell adhesion, proliferation, apoptosis, and cell cycle ratio as well as their differentiation potential were evaluated. The data showed that all of these parameters were significantly different when comparing serum-free cultures with and without 10 nM/L EGF (p < 0.05/0.01); however, cells with 10 nM/L EGF did not respond differently than cells grown in standard serum-containing media without EGF (p > 0.05). Serum-free medium was created by adding different components to basal medium ultra-CULTURE as specified in the Materials and Methods, under the heading; Optimization of culture conditions for mp-BM-MSCs. ultra-CULTURE is a commercially available product. The precise formula is proprietary. https://link.springer.com/content/pdf/10.1007%2Fs11626-013-9665-6.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |