Cell line/type | hESC-derived neural precursors |
---|---|
Species | Human |
Animal free | Yes |
Product | GRM supplemented |
Erceg, S., Laínez, S., Ronaghi, M., Stojkovic, P., Pérez-Aragó, M. A., Moreno-Manzano, V., ... & Stojkovic, M. (2008). Differentiation of human embryonic stem cells to regional specific neural precursors in chemically defined medium conditions. PLoS One, 3(5), e2122. In this study, the cells of two different hESC lines were converted to neural rosettes using adherent and chemically defined conditions. The progenitor cells were exposed to retinoic acid (RA) or to human recombinant basic fibroblast growth factor (bFGF) in the late phase of the rosette formation. Exposing the progenitor cells to RA suppressed differentiation to rostral forebrain dopamine neural lineage and promoted that of spinal neural tissue including motor neurons. The functional characteristics of these differentiated neuronal precursors under both, rostral (bFGF) and caudalizing (RA) signals were confirmed by patch clamp analysis. These findings suggest that this differentiation protocol has the capacity to generate region-specific and electrophysiologically active neurons under in vitro conditions without embryoid body formation, co-culture with stromal cells and without presence of cells of mesodermal or endodermal lineages. GRM medium was supplemented with different components as specified in the Materials and Methods; under the header Neural Differentiation. The formulation of GRM medium is listed in the Materials and Methods, under the header Neural Differentiation. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2346555/pdf/pone.0002122.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |