Cell line/type | hES-derived TH1 cells |
---|---|
Species | Human |
Animal free | Yes |
Product | N2 medium |
Vallier, L., & Pedersen, R. (2008). Differentiation of human embryonic stem cells in adherent and in chemically defined culture conditions. Current protocols in stem cell biology, 4(1), 1D-4. In this study, culture conditions to differentiate pluripotent cells in adherent conditions and in the absence of stroma cells, feeder cells, conditioned medium, serum, or complex matrices are described. Importantly, these defined culture conditions are devoid of animal products, thereby eliminating factors that could obscure analysis of developmental mechanisms or render the resulting tissues incompatible with future clinical applications. A multiple-step culture method was modified to improve the differentiation efficiency. According to the experimental results, the human ES cell line, PKU1.1, which has maintained in an undifferentiated state for 102 passages could directly differentiate into THþ neurons in a chemically defined system by several steps: the generation of EBs (Stage 2), selection and proliferation for NPCs (Stage 3) and differentiation of the progenitor cells to THþ cells (Stage 4). The formulation and supplementation of N2 medium is specified in the Materials and Methods, under the headings; Differentiation and enrichment of NPCs and TH1 cell differentiation. https://currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/9780470151808.sc01d04s4 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |