Cell line/type | Hippocampal neurons |
---|---|
Species | Rat |
Animal free | Yes |
Product | Neurobasal-A Edwards et al. 2010 |
Edwards, D., Das, M., Molnar, P., & Hickman, J. J. (2010). Addition of glutamate to serum-free culture promotes recovery of electrical activity in adult hippocampal neurons in vitro. Journal of neuroscience methods, 190(2), 155-163. In this study, the authors report on the development of a defined culture system with the ability to support functional recovery and long-term survival of adult rat hippocampal neurons. In this system, the cell-adhesive substrate, N-1 [3-(trimethoxysilyl) propyl]-diethylenetriamine, supported neuronal attachment, regeneration, and long-term survival of adult neurons for more than 80 days in vitro. The culture medium was composed of Neurobasal-A supplemented with different components as specified in the Material and Methods, under the heading; 2.3. Isolation and culture of adult hippocampal neurons. https://www.sciencedirect.com/science/article/pii/S0165027010002359 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |