Cell line/type | mESCs derived Flk-1 + vascular progenitor cells |
---|---|
Species | Mouse |
Animal free | No |
Product | NS1D2b |
Blancas, A. A., Shih, A. J., Lauer, N. E., & McCloskey, K. E. (2011). Endothelial cells from embryonic stem cells in a chemically defined medium. Stem cells and development, 20(12), 2153-2161. In this study, the development of new medium formulations for deriving ECs from murine embryonic stem cells (mESCs) using only chemically defined reagents was explored. Two different medium formulations were presented along with the detailed methodologies, including the optimization of extracellular matrix–derived substrates known to play a role in cell attachment and proliferation as well as cell differentiation. Characterization of the ESC-derived ECs indicate that (1) chemically defined medium formulations reproducibly generate superior ECs compared with previous serum-containing formulations, (2) fibronectin, and not collagen type-IV, is the optimal substrate for EC induction in our chemically defined medium formulations, (3) without additional activation of Notchsignaling, ESC-ECs develop predominantly into venous ECs, and (4) using these medium formulations, a second rigorous selection step is not required to generate proliferating ECs from ESCs, but it does enhance the final purity of the ECs The first induction medium optimized for the generation of Flk-1 + vascular progenitor cells is called ‘‘NS1D2b.’’ The formulation of NS1D2b is listed in Table 1. Chemically Defined Medium Formulations for the Derivation of Endothelial Cell from Murine Embryonic Stem Cell. The second medium, ‘‘LDSk’’ is optimized for EC specification and expansion. The formulation of LDSk is listed in Table 1. Chemically Defined Medium Formulations for the Derivation of Endothelial Cell from Murine Embryonic Stem Cell. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3225059/pdf/scd.2010.0432.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |
Contains phenol red > Yes | Yes |