Cell line/type | hESC-derived endothelial cells |
---|---|
Species | Human |
Animal free | Yes |
Product | APEL medium Costa et al. 2013 |
Costa, M., Sourris, K., Lim, S. M., Qing, C. Y., Hirst, C. E., Parkington, H. C., ... & Koutsis, K. (2013). Derivation of endothelial cells from human embryonic stem cells in fully defined medium enables identification of lysophosphatidic acid and platelet activating factor as regulators of eNOS localization. Stem cell research, 10(1), 103-117. This study aimed to generate endothelial progenitors from human pluripotent stem cells to facilitate the study of human EC physiology, using a defined serum-free protocol. Human embryonic stem cells differentiated under serum-free conditions generated CD34(+)KDR(+) endothelial progenitor cells after 6days that could be further expanded in the presence of vascular endothelial growth factor. The culture medium used in this study is APEL medium, with modifications which are specified in the Methods, under the header; hESC culture and differentiation. The formulation of APEL medium is listed in a previous article, Ng et al. 2008. Ng, E.S., Davis, R., Stanley, E.G., Elefanty, A.G., 2008. A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies. Nat. Protoc. 3 (5), 768–776. https://ac.els-cdn.com/S1873506112001055/1-s2.0-S1873506112001055-main.pdf?_tid=1e76b85b-32ad-4b1e-a3b3-5e3dd00981c3&acdnat=1530020685_037531f3b90cefe724b2b8b7b3d2e47b |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |