Cell line/type | hESC-derived endothelial cells |
---|---|
Species | Human |
Animal free | Yes |
Product | CD-U3 supplemented |
Jordan, I., Horn, D., John, K., & Sandig, V. (2013). A genotype of modified vaccinia Ankara (MVA) that facilitates replication in suspension cultures in chemically defined medium. Viruses, 5(1), 321-339. In this study, to facilitate vaccine programs utilizing host-restricted poxviruses, avian suspension cell lines (CR and CR.pIX) was established and a robust, chemically defined, culturing process for production of this class of vectors was developed. For one prominent member, modified vaccinia Ankara (MVA), a new strain is described that appears to replicate to greater yields of infectious units, especially in the cell-free supernatant of cultures in chemically defined media. Chemically defined CD-U3 medium is an improved version of CD-U2, supplemented with glutamine, alanine (both 2 mM) and recombinant insulin-like growth factor (LONG-R3 IGF, 10 ng/mL).CD-U3 medium is developed by PAA Laboratories and is a proprietary medium. https://res.mdpi.com/def50200b70fc69beb0499345a01332dbffc4718e54f3ca3c1a6f951daa2c22da9a1b7502848d3fe6aefdddd5e36b528e558439ed9eab21f1702924d5ee22fbfa7a61fd485f58a2b7e6a0ab1dae4d0057de3e1689d33f939991adb0aeebece4a943dea6c3257a19c94b207fbbc5a68bd0acf81bbce |
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Source | Literature - own formulation |
Contains phenol red > Yes | Yes |