Cell line/type | hPSCs-derived neuroepithelial cells |
---|---|
Species | Human |
Animal free | Yes |
Product | E6 medium |
Lippmann, E. S., Estevez‐Silva, M. C., & Ashton, R. S. (2014). Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation without small molecule inhibitors. Stem Cells, 32(4), 1032-1042. In this study, hPSCs were maintained under chemically defined, feeder-independent, and xeno-free conditions. hPSCs can be directly differentiated into pure neuroepithelial cultures ([mt]90% Pax6(+)/N-cadherin(+) with widespread rosette formation) within 6 days under adherent conditions, without small molecule inhibitors, and using only minimalistic medium consisting of Dulbecco's modified Eagle's medium/F-12, sodium bicarbonate, selenium, ascorbic acid, transferrin, and insulin (i.e., E6 medium). Evidence is provided that the defined culture conditions enable this high level of neural conversion in contrast to hPSCs maintained on mouse embryonic fibroblasts (MEFs). In addition, hPSCs previously maintained on MEFs could be rapidly converted to a neural compliant state upon transfer to these defined conditions while still maintaining their ability to generate all three germ layers. Overall, this fully defined and scalable protocol should be broadly useful for generating therapeutic neural cells for regenerative applications. E6 medium was supplemented with different components as specified in the Materials and Methods, under the heading; Differentiation to Neuroepithelium Under Adherent Conditions. E6 medium is the same formulation as E8 medium but without FGF2 and TGFb1.The formulation and preparation protocol of E8 medium is listed in a previous article, Lippmann et al. 2014. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |