Cell line/type | TS (trophoblast stem) cells |
---|---|
Species | Mouse |
Animal free | Yes |
Product | TX Medium |
Kubaczka, C., Senner, C., Araúzo-Bravo, M. J., Sharma, N., Kuckenberg, P., Becker, A., ... & Schorle, H. (2014). Derivation and maintenance of murine trophoblast stem cells under defined conditions. Stem cell reports, 2(2), 232-242. DOI: 10.1016/j.stemcr.2013.12.013. In this study, a simple medium is developed consisting of ten chemically defined ingredients for culture of TSCs on Matrigel or synthetic substrates, named TX medium. Gene expression and DNA methylation profiling demonstrated the faithful propagation of expression profiles and epigenomic characteristics of TSCs cultured in TX. Further, TX medium supported the de novo derivation of TSC lines. Finally, TSCs cultured in TX differentiate into all derivatives of the trophectodermal lineage in vitro, give rise to hemorrhagic lesions in nude mice, and chimerize the placenta, indicating that they retained all hallmarks of TSCs. TX media formulation no longer requires fetal bovine serum and conditioned medium, which facilitates and standardizes the culture of this extraembryonic lineage. TX medium is modified TS medium, as described in the Experimental Procedures, under the heading; Cell Culture. The formulation of TS-medium can be found online-->http://cshprotocols.cshlp.org/content/2006/1/pdb.rec10416.full?text_only=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923226/pdf/main.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |