Cell line/type | TS (trophoblast stem) cells |
---|---|
Species | Mouse |
Animal free | No |
Product | CDM/FAXY |
Ohinata, Y., & Tsukiyama, T. (2014). Establishment of trophoblast stem cells under defined culture conditions in mice. PloS one, 9(9), e10730. This study reports that addition of FGF2, activin A, XAV939, and Y27632 are necessary and sufficient for derivation of TS (trophoblast stem) cells from both of E3.5 blastocysts and E6.5 early postimplantation extraembryonic ectoderm. Moreover, the undifferentiated TS cell state can be stably maintained in chemically defined culture conditions. Cells derived in this manner expressed TS cell marker genes, including Eomes, Elf5, Cdx2, Klf5, Cdh1, Esrrb, Sox2, and Tcfap2c; differentiated into all trophoblast subtypes (trophoblast giant cells, spongiotrophoblast, and labyrinthine trophoblast) in vitro; and exclusively contributed to trophoblast lineages in chimeric animals. This delineation of minimal requirements for derivation and self-renewal provides a defined platform for precise description and dissection of the molecular state of TS cells. The formulation of CDM/FAXY medium is listed in the Materials and Methods, under the heading; TS cell derivation. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159327/pdf/pone.0107308.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |