Cell line/type | hASC-HUVEC (co-culture of human adipose stromal cells, human umbilical vein endothelial cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | VSM |
Huttala, O., Vuorenpää, H., Toimela, T., Uotila, J., Kuokkanen, H., Ylikomi, T., ... & Heinonen, T. (2015). Human vascular model with defined stimulation medium-a characterization study. In this work a defined serum-free vascular stimulation medium (VSM) was developed. Further characterization was performed in terms of cell identity, maturation and structure to obtain a thoroughly characterized in vitro vascular model to replace or reduce corresponding animal experiments. The results showed that the novel vascular stimulation medium induced intact and evenly distributed vascular-like network with morphology of mature vessels. Electron microscopic analysis assured the three-dimensional microstructure of the network containing lumen. Additionally, elevated expressions of the main human angiogenesis-related genes were detected. In conclusion, with the new defined medium the vascular model can be utilized as a characterized test system for chemical testing as well as in creating vascularized tissue models. The formulation of VSM media is listed in Tab. 1: Stimulation media tested in vascular model. https://www.altex.org/index.php/altex/article/download/233/238 |
|
Source | Literature - own formulation |
Antibiotics free > Yes | Yes |
Contains phenol red > Yes | Yes |