Cell line/type | Hepatoblasts |
---|---|
Species | Mouse |
Animal free | Yes |
Product | DMEM/F12 supplemented Lv et al. 2015 |
Lv, L., Han, Q., Chu, Y., Zhang, M., Sun, L., Wei, W., ... & Li, W. (2015). Selfârenewal of hepatoblasts under chemically defined conditions by iterative growth factor and chemical screening. Hepatology, 61(1), 337-347. In this study, the authors devised a screening strategy aimed at identifying growth factors and small molecules that can sustain self-renewal of mouse hepatoblasts. This approach began with a defined basal condition, on top of which collections of growth factors and bioactive small molecules were screened for maintaining self-renewal of primary hepatoblasts. The initially identified proteins and small molecules were then combined in the basal media for subsequent screening to identify additional molecules that can synergistically promote hepatoblast self-renewal. This strategy was performed iteratively to eventually define a small molecule and growth factor cocktail, including epidermal growth factor, glycogen synthase kinase 3 inhibitor, transforming growth factor beta receptor inhibitor, lysophosphatidic acid, and sphingosine 1-phosphate, which was sufficient to sustain long-term self-renewal of the murine hepatoblasts under chemically defined conditions. These expanded hepatoblasts retain the ability to respond to liver developmental cues and produce functional hepatocytes and form bile duct-like structures. In this culture system, the only undefined components are BSA and Matrigel. It was found that laminin (a major component of Matrigel) and human recombinant albumin (ALB) can replace Matrigel and BSA, respectively, without compromising the derivation and long-term self-renewal of eHBs. The precise formulation of the culture medium is listed in the Materials and Methods, under the heading; Cell Isolation and Growth Factor/Small Molecule Screens. https://aasldpubs.onlinelibrary.wiley.com/doi/pdf/10.1002/hep.27421 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |