Cell line/type | Chondrocytes |
---|---|
Species | Bovine (cow) |
Animal free | No |
Product | SF3D Ahmed et al. 2014 |
Ahmed, N., Iu, J., Brown, C. E., Taylor, D. W., & Kandel, R. A. (2014). Serum-and growth-factor-free three-dimensional culture system supports cartilage tissue formation by promoting collagen synthesis via Sox9–Col2a1 interaction. Tissue Engineering Part A, 20(15-16), 2224-2233. In this study, a novel culture method is described that allows for the increase in the number of chondrocytes and promotes hyaline-like cartilage tissue formation in part by insulin-mediated Sox9-Col2a1 binding. Bovine articular chondrocytes were passaged twice to allow for cell number expansion (P2) and cultured at high density on 3D collagen-type-II-coated membranes in high glucose content media supplemented with insulin and dexamethasone (SF3D). The formulation of SF3D medium is listed in the Materials and Methods, under the heading; Cell isolation and culture. https://www.liebertpub.com/doi/pdfplus/10.1089/ten.tea.2013.0559 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |