Cell line/type | Chondrocytes |
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Species | Human |
Animal free | Yes |
Product | high-glucose DMEM Koo et al. 2010 |
Koo, J., Kim, K. I., Min, B. H., & Lee, G. M. (2010). Controlling medium osmolality improves the expansion of human articular chondrocytes in serum-free media. Tissue Engineering Part C: Methods, 16(5), 957-963. The aim of this study was to investigate the effects of medium osmolality on the expansion of human articular chondrocytes (HACs) with serum-free media (SFM), proprietary SFM of various osmolalities (290, 320, 350, 400, and 450 mOsm/kg), supplemented with components known to enhance chondrocyte growth, were constructed by the adjustment of NaCl concentration. Taken together, these results show that the expansion of HACs in SF cultures can be improved by adjusting the medium osmolality to be within the range of 290-350 mOsm/kg and that controlling medium osmolality during monolayer cultures does not deter the tissue-forming capability of the cells. The basal medium was a high-glucose DMEM containing 4.5 g=L of glucose, modified to exclude NaCl normally formulated into the basal medium, to enable the construction of SFM at various osmolalities. SFM made in the customized DMEM without NaCl resulted in SFM with an osmolality of approximately 191mOsm=kg. Various amounts of 5M NaCl were then added to the SFM to construct a total of five SFM with osmolalities of 290, 320, 350, 400, and 450 mOsm=kg, respectively. The formulation of the modified high-glucose DMEM based medium is listed in the Materials and Methods, under the heading; Preparation of expansion SFM at various osmolalities. https://www.liebertpub.com/doi/pdfplus/10.1089/ten.tec.2009.0525 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |