Cell line/type | Chondrocytes |
---|---|
Species | Human |
Animal free | Yes |
Product | H- F+ I+ C' medium |
Iwata, K., Asawa, Y., Nishizawa, S., Mori, Y., Nagata, S., Takato, T., & Hoshi, K. (2012). The development of a serum-free medium utilizing the interaction between growth factors and biomaterials. Biomaterials, 33(2), 444-454. The aim of this study was to develop a serum-free medium that would increase the cell numbers by more than 20-fold within one week, which proliferation ability almost matches that of serum-based one. From various growth factors that are contained within the serum, a cocktail was made of FGF-2 (100 ng/mL), insulin (5 mug/mL), EGF (10 pg/mL), PDGF (625 pg/mL) and TGF-beta (5 pg/mL), which increased the chondrocyte numbers by approximately 3-fold for 7 days. Moreover, the biomaterials including albumin and hyaluronan were used as the carrier of those factors. By direct mixing of those factors with biomaterials before the administration to the medium, the medium containing that mixture showed the chondrocyte growth of approximately a 25-fold increase by day 10. In this medium, the FGF-2 or insulin concentration hardly decreased, and rather enhanced the activation of ERK. Due to the optimal usage of biomaterials, this serum-free medium will realize a constant harvest of chondrocytes and could contribute to the safety and quality in regenerative medicine. The formulation of the serum-free H- F+ I+ C' medium is listed in the Results, under the heading; 3.1. Optimization of medium content. https://ac.els-cdn.com/S014296121101132X/1-s2.0-S014296121101132X-main.pdf?_tid=ad9b6ba6-9300-4857-ab91-dc9e83137021&acdnat=1531907229_3a210e49c37c560cf28e5e6723cf0d4e |
|
Source | Literature - own formulation |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |