Cell line/type | Chondrocytes |
---|---|
Species | Porcine (Pig) |
Animal free | Yes |
Product | F12 + growth factors |
Kamil, S. H., Kojima, K., Vacanti, M. P., Zaporojan, V., Vacanti, C. A., & Eavey, R. D. (2007). Tissue engineered cartilage: utilization of autologous serum and serum-free media for chondrocyte culture. International journal of pediatric otorhinolaryngology, 71(1), 71-75. DOI: 10.1016/j.ijporl.2006.09.002 In this study, the potential of chondrocyte expansion using autologous and serum-free media was evaluated. Autologous auricular cartilage was harvested in a swine model. An initial concentration of 100x10(3) cells per group were expanded in three groups. Group A, F-12 with 10% fetal calf serum; Group B, F-12 supplemented with 10% autologous serum; Group C, F-12 supplemented with growth factors. The cells in all the three groups exhibited normal chondrocyte morphology. The results suggest that both autologous serum as well as serum-free media might be substituted for the expansion of the number of chondrocytes, thus avoiding the potential need for a bovine serum supplement. The composition of Group C = Ham F12 culture medium supplemented with growth factors is listed in the Materials and Methos, under the heading; 2.3. In vitro cell cultures medium. https://ac.els-cdn.com/S0165587606003454/1-s2.0-S0165587606003454-main.pdf?_tid=7b1803a7-0f31-489c-8a74-b2dfa9f0538b&acdnat=1534430149_1d18e3cad2f393fe32734307342f92c8 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |