Cell line/type | Cerebral organoids |
---|---|
Species | Human |
Animal free | Yes |
Product | E8 Lindborg et al. 2016 |
Lindborg, B. A., Brekke, J. H., Vegoe, A. L., Ulrich, C. B., Haider, K. T., Subramaniam, S., ... & Wang, Q. (2016). Rapid induction of cerebral organoids from human induced pluripotent stem cells using a chemically defined hydrogel and defined cell culture medium. Stem cells translational medicine, 5(7), 970-979. In this study, a robust and simple method for generating cerebral organoids from human induced pluripotent stem cells by using a chemically defined hydrogel material and chemically defined culture medium is described. This method, by virtue of its simplicity and use of defined materials, greatly facilitates access to cerebral organoid technology, enables scalable applications, and provides a potential pathway to translational applications where defined components are desirable. Using hPSCs maintained in chemically defined E8 medium on either Matrigel-coated or recombinant human vitronectin peptide (VTN-NC)-coated substrates, the authors demonstrate a procedure where hPSCs can be differentiated into neuroepithelium with high purity after only 6 days and without the presence of small molecule SMAD inhibitors. The formulation and preparation protocol of E8 medium are listed in a previous article, Lippmann et al. 2014. Lippmann, E. S., EstevezāSilva, M. C., & Ashton, R. S. (2014). Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation without small molecule inhibitors. Stem Cells, 32(4), 1032-1042. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4922855/pdf/sctm_20150305.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |