Cell line/type | Microglia |
---|---|
Species | Rat |
Animal free | Yes |
Product | Astrocyte conditioned medium |
Bohlen, C. J., Bennett, F. C., Tucker, A. F., Collins, H. Y., Mulinyawe, S. B., & Barres, B. A. (2017). Diverse requirements for microglial survival, specification, and function revealed by defined-medium cultures. Neuron, 94(4), 759-773. In this study, serum-free culture conditions were optimized to permit robust survival of highly ramified adult microglia under defined-medium conditions. The authors found that astrocyte-derived factors prevent microglial death ex vivo and that this activity results from three primary components, CSF-1/IL-34, TGF-beta2, and cholesterol. Using microglial cultures that have never been exposed to serum, a dramatic and lasting change in phagocytic capacity after serum exposure was demonstrated. Mature microglia rapidly lose signature gene expression after isolation, and that this loss can be reversed by engrafting cells back into an intact CNS environment. These data indicate that the specialized gene expression profile of mature microglia requires continuous instructive signaling from the intact CNS. Growth medium that had been previously exposed to dense astrocyte cultures for three days (ACM) was sufficient to sustain microglial survival in the absence of serum, suggesting that astrocytes secrete factors that promote microglial survival. The preparation of astrocyte conditioned medium (ACM) is specified in the Method Detail. The formulation of Base microglial growth medium (MGM) is listed in the EXPERIMENTAL MODEL AND SUBJECT DETAIL of the STAR+METHODS, under the heading, Rat microglia culture. https://www.sciencedirect.com/science/article/pii/S0896627317304002 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |