Carmean, N., Kosman, J. W., Leaf, E. M., Hudson, A. E., Opheim, K. E., & Bassuk, J. A. (2007). Immortalization of human urothelial cells by human papillomavirus type 16 E6 and E7 genes in a defined serumāfree system. Cell proliferation, 40(2), 166-184.
DOI: 10.1111/j.1365-2184.2007.00428.x
In this study, a procedure for the immortalization of urothelial cells using a defined, serum-free culture system was developed.
DK-SFM + GS medium is a 50/50 (v/v) mixture of DMEM and Defined Keratinocyte Serum Free Medium supplemented with the manufacturer’s growth supplement. The composition is described in the Materials and Methods, under the heading; Cell Culture. The DK-SFM + GS medium includes commercial products.
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