Cell line/type | SSCs (Spermatogonial stem cells) |
---|---|
Species | Mouse |
Animal free | No |
Product | IMDM Kanatsu-Shinohara et al. 2014 |
Kanatsu-Shinohara, M., Ogonuki, N., Matoba, S., Morimoto, H., Ogura, A., & Shinohara, T. (2014). Improved serum-and feeder-free culture of mouse germline stem cells. Biology of reproduction, 91(4), 88-1. In this study, a novel culture medium is reported that showed improved spermatogonial stem cells (SSCs) maintenance. Iscove modified Dulbecco medium, supplemented with lipid mixture, fetuin, and knockout serum replacement was used. In the presence of glial cell line-derived neurotrophic factor and fibroblast growth factor 2, SSCs cultured on laminin-coated plates could proliferate for more than 5 mo and maintained normal karyotype and androgenetic DNA methylation patterns in imprinted genes. Germ cell transplantation showed that SSCs in the serum-free medium proliferated more actively than those in the serum-supplemented medium and that the frequency of SSCs was comparable between the two culture media. Cultured cells underwent germline transmission. The formulation of the IMDM based culture medium is listed in the Supplemental Table S1. https://academic.oup.com/biolreprod/article/91/4/88,%201-11/2434297 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |