Cell line/type | SSCs (Spermatogonial stem cells) |
---|---|
Species | Mouse |
Animal free | No |
Product | Knockout SR medium |
Wang, Q. Z., & Hna, C. S. (2008). Serum-free knockout SR medium supports the short-time viability of mouse spermatogonial stem cells. Fen zi xi bao sheng wu xue bao= Journal of molecular cell biology, 41(2), 162-166. In this study, the authors selected the mouse spermatogonial stem cells (SSCs) from mice on STO (SIM mouse embryo-derived thioguanine and ouabain resistant) feeders by differential adherence selection in DMEM/F12 containing 5% FBS, and used a serum-free defined medium prepared with Knockout SR basal medium to culture SSCs. The results showed that enriched SSCs could be maintained for a short of time and form colonies, but the proliferation of SSCs was unconspicuous, suggesting that some factors that are detrimental to the self-renewal of SSCs possibly existed in the Knockout SR basal medium. Knockout SR is a commercial product. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pubmed/18567507 |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |