Cell line/type | Umbilical cord blood stem cells |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM/F12 Ali et al. 2009 |
Ali, H., Jurga, M., Kurgonaite, K., Forraz, N., & McGuckin, C. (2009). Defined serum-free culturing conditions for neural tissue engineering of human cord blood stem cells. Acta neurobiologiae experimentalis, 69(1), 12-23. The authors previously developed a three-step protocol for isolation, expansion and sequential neuronal differentiation of cord blood pluripotent stem cells (characterized with our unique triple immunocytochemisty scheme for Oct-4, Sox-2 and Nanog) in defined serum-free culturing conditions. In this study, the authors incorporated this protocol with 3-dimensional culturing systems which produced artificial neuronal tissues expressing Nestin, NF-200, TUJ1, PSD-95 and NeuN. The formulation of the culture medium is listed in Table 1 & 2. http://www.ane.pl/linkout.php?pii=6902 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |