|Cell line/type||Nucleus Pulposus cells|
Reza, A. T., & Nicoll, S. B. (2010). Serum‐free, chemically defined medium with TGF‐β3 enhances functional properties of nucleus pulposus cell‐laden carboxymethylcellulose hydrogel constructs. Biotechnology and bioengineering, 105(2), 384-395.
In this study, a standard serum-containing medium formulation was compared to a chemically defined, serum-free medium to determine the effect on matrix elaboration and functional properties of nucleus pulposus (NP) cell-laden carboxymethylcellulose (CMC) hydrogels. Additionally, both media were further supplemented with transforming growth factor-beta 3 (TGF-beta(3)). The results demonstrate enhanced functional matrix development by NP cells when cultured in CMC hydrogels maintained in serum-free, TGF-b3 supplemented medium, indicating the importance of medium formulation in NP construct development.
The formulation of chemically defined, serum-free CDM+ medium is listed in the Materials and Methods, under the heading; Cell Encapsulation in Photocrosslinked Hydrogels.
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > Yes||Yes|
|Antibiotics free > No||No|