Cell line/type | D3 mESC line |
---|---|
Species | Mouse |
Animal free | No |
Product | Modified Advanced™ DMEM |
Riebeling, C., Schlechter, K., Buesen, R., Spielmann, H., Luch, A., & Seiler, A. (2011). Defined culture medium for stem cell differentiation: applicability of serum-free conditions in the mouse embryonic stem cell test. Toxicology in Vitro, 25(4), 914-921. In this study the serum-free medium and feeder cell-free maintenance of the mouse embryonic stem cell line D3 and investigate the use of specific growth factors for induction of cardiac differentiation is demonstrated. Using a combination of bone morphogenetic protein-2, bone morphogenetic protein-4, activin A and ascorbic acid, embryoid bodies efficiently differentiated into contracting myocardium. Additionally, examining levels of intracellular marker proteins by flow cytometry not only confirmed differentiation into cardiomyocytes, but demonstrated significant differentiation into neuronal cells in the same time frame. Thus, this approach might allow for simultaneous detection of developmental effects on both early mesodermal and neuroectodermal differentiation. The serum-free conditions for maintenance and differentiation of D3 cells described here enhance the transferability and standardisation and hence the performance of the EST. Advanced™ DMEM was supplemented with different components as specified in the Materials and Methods, under the heading; 2.2. Cell culture. The formulation of base medium advanced DMEM can be found online: Advanced DMEM: http://www.thermofisher.com/nl/en/home/technical-resources/media-formulation.1.html https://www.sciencedirect.com/science/article/pii/S0887233311000555 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |
Contains phenol red > Yes | Yes |