Cell line/type | UC-WJ (umbilical cord Wharton's jelly) cells |
---|---|
Species | Bovine (cow) |
Animal free | Unspecified |
Product | Stemline® Mesenchymal Stem Cell Expansion Medium |
Cardoso, T. C., Ferrari, H. F., Garcia, A. F., Novais, J. B., Silva-Frade, C., Ferrarezi, M. C., ... & Gameiro, R. (2012). Isolation and characterization of Wharton’s jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system. BMC biotechnology, 12(1), 18. In this study, bovine-derived umbilical cord Wharton's jelly (UC-WJ) cells were isolated, characterized and maintained under 3D-free serum condition as an alternative of stem cell source for future cell banking. Bovine-derived UC-WJ cells, collected individually from 5 different umbilical cords sources, were successfully cultured under serum-free conditions and were capable to support 60 consecutive passages using commercial Stemline((R)) mesenchymal stem cells expansion medium. Moreover, the UC-WJ cells were differentiated into osteocytes, chondrocytes, adipocytes and neural-like cells and cultured separately. Stemline® Mesenchymal Stem Cell Expansion Medium is a commercial medium. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3443425/pdf/1472-6750-12-18.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |