Cell line/type | R1-Oct4-EGFP mESC line |
---|---|
Species | Mouse |
Animal free | No |
Product | KSR-HNF |
Liu, L., Yuan, Q., Shi, J., Li, X., Jung, D., Wang, L., ... & Chen, Y. (2012). Chemically-defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions. Biotechnology letters, 34(10), 1951-1957. In this study mouse ESC (mESC) were cultured on electrospun nanofibers made from polymethylglutarimide (PMGI), which is a synthetic thermoplastic polymer stable under culture conditions. mESCs on nanofibers had a growth rate comparable to those cultured conventionally and retained their pluripotency. Furthermore, self-renewed ESCs differentiated into all three germ layers thereby providing a reliable way to expand mESCs without feeder cells. mESCs could attach and grow on HNF in KSR-supplemented medium for over 20 passages and still strongly expressed the SSEA-1 pluripotency marker at passage 3. KSR (KnockOut Serum replacement) is a commercial product. The precise formulation of the culture medium is not included. https://link.springer.com/content/pdf/10.1007%2Fs10529-012-0973-9.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |