Cell line/type | Cartilage |
---|---|
Species | Bovine (cow) |
Animal free | Yes |
Product | CDM + TGF-b3 |
Byers, B. A., Mauck, R. L., Chiang, I. E., & Tuan, R. S. (2008). Transient exposure to transforming growth factor beta 3 under serum-free conditions enhances the biomechanical and biochemical maturation of tissue-engineered cartilage. Tissue Engineering Part A, 14(11), 1821-1834. doi: 10.1089/ten.tea.2007.0222. This study details a systematic characterization of a serum-free (SF) culture methodology employing transient growth factor supplementation to promote robust maturation of tissue-engineered cartilage. Bovine chondrocyte agarose hydrogel constructs were cultured under free-swelling conditions in serum-containing or SF medium supplemented continuously or transiently with varying doses of transforming growth factor beta 3 (TGF-beta3). Transient exposure (2 weeks) to low concentrations (2.5-5 ng/mL) of TGF-beta3 in chemically defined medium facilitated robust and highly reproducible construct maturation. Constructs receiving transient TGF-beta3 exposure achieved native tissue levels of compressive modulus (0.8 MPa) and proteoglycan content (6-7% of wet weight) after less than 2 months of in vitro culture. This maturation response was far superior to that observed after continuous growth factor supplementation or transient TGF-beta3 treatment in the presence of serum. The formulation of the chemically-defined medium is listed in the Materials and Methods, under the heading; Cell isolation and construct fabrication. https://www.liebertpub.com/doi/pdfplus/10.1089/ten.tea.2007.0222 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |