Cell line/type | Cartilage |
---|---|
Species | Rabbit |
Animal free | Yes |
Product | Chondrogenic medium |
Whitney, G. A., Mera, H., Weidenbecher, M., Awadallah, A., Mansour, J. M., & Dennis, J. E. (2012). Methods for producing scaffold-free engineered cartilage sheets from auricular and articular chondrocyte cell sources and attachment to porous tantalum. BioResearch open access, 1(4), 157-165. In this study, methods for cartilage construct generation from auricular and articular cell sources are described in detail, and the resulting constructs are compared for use in a joint resurfacing model. Attachment of cartilage sheets to porous tantalum is also investigated as a potential vehicle for future attachment to subchondral bone. Large scaffold-free cartilage constructs were produced from culture-expanded chondrocytes from skeletally mature rabbits, and redifferentiated in a chemically-defined culture medium. The obtained results demonstrate that large scaffold-free neocartilage constructs can be produced from mature culture-expanded chondrocytes in a chemically-defined medium, and that these constructs can be attached to porous tantalum. The formulation of the chondrogenic defined medium is listed in the Materials and Methods, under the heading; Scaffold-free construct formation. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3559237/pdf/biores.2012.0231.pdf |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |